The authors examine whether an analysis framework, the Mathematical Quality of Instruction (MQI), can assist prospective teachers in noticing and interpreting salient aspects of classroom practice. The findings indicate that participation in a video club led prospective teachers to be better able to notice these salient features of mathematics instruction when watching video-taped lessons. Furthermore, participants adopted a more interpretive stance toward the classroom components they noticed and used evidence to support their stance. Prospective teachers’ abilities to notice students’ mathematical thinking improved as a result of structured video analysis.